Part:BBa_I716017:Design

yfbE + (rbs-RFP)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 303
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 886
  Illegal AgeI site found at 998
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

composite <yfbE promoter><rbs><RFP>
[digest] I716014 (yfbE solo)                 (EcoRI/BamHI, 2063+ 317, S)
[digest] pBca1100-Bca1109                    (EcoRI/BamHI, 2927+1253, L)
[ligate] pBca9145-yfbE_pro-rbs-RFP (I716017)

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Source

I716014 + pBca1100-Bca1109